APPLIKATIONER
Liste over udsendte applikationer.
Repromer HPLC kolonner fra Dr. Maisch
POLYGEN præsenterer en række nyudviklede HPLC kolonner fra Dr. Maisch, Tyskland:
Repromer
Katalog over disse Repromer kolonner med beskrivelser, applikationer, liste over retentionstid samt en krydsliste over konkurrent kolonner og tilsvarende Repromer kolonner kan hentes her i PDF format.
Tilbud, yderligere oplysninger samt priser kan indhentes her info@polygen.dk
Vi tilbyder disse kolonner til meget konkurrencedygtige priser samt tilbyder afprøvning i en periode af 14 dage – uforpligtende – og kan sendes retur, såfremt kolonne ikke giver tilfredsstillende resultater.
What are preparative, semipreparative and analytical HPLC?
Please help me in understanding the exact differences between analytical HPLC, semipreparative HPLC and preparative HPLC
Analytical HPLC is usually carried out under high backpressures. For example in Reverse-Phase High Performance Liquid Chromatography by using 250 cm length x 4.6 mm Inner Diameter, and 5 micras C18 columns, and flow rates around 0.5 ml/min of solvents like Acetonitrile (low viscosity) and temperatures about 20 or 30ºC, the minimum backpressure recommended is 11 bar.
Otherwise, preparative and semipreparative chromatography are carried out under low backpressures (1-5 bar) with bigger inner diameter columns and bigger particle size (10 micras in some cases) by passing mobile phases at high flow rates (4-5 ml/min). Alternatively, you can accomplished preparative and semipreparative chromatography by using a short inner diameter and small size particle (5 micras) by passing a very small flow rate (about 0.05 ml/min) mobile phases with very low viscosity (hexane for example) in the aim of keeping a very low backpressure and saving solvents.
In addition, the most important difference between preparative/semipreparative and analytical HPLC is the purpose. Whereas the first one is designed for obtaining the fractionation of groups of compounds (for example fractionation of PAHs and oxy-PAHs) usually as a function of their polarity, analytical HPLC is designed for separation of individual compounds as a function of their medium polarizabilities.
Maybe these two articles can help understanding the mechanisms of both methodologies.

Clinical (hospital) centrifuger

TurboCycler 2 gradient contra SensoQuest LabCycler Gradient
GPC/SEC Tips&Tricks 12/2017
Polymer Standard Service (PSS) udgiver jævnligt artikler indenfor GPC/SEC Tips & Tricks sammen med LC/GC Europe i bladet LCGC Europe`s The Column. Sidste udgivelse er “How to Treat Your RI Detector“
GPC/SEC Tips & Tricks
Polymer Standard Service (PSS) udgiver jævnligt artikler indenfor GPC/SEC Tips & Tricks sammen med LC/GC Europe i bladet LCGC Europe`s The Column. Sidste udgivelse er “Inter-Detector Delay“
Ny artikel indenfor GPC/SEC Tips & Tricks
Polymer Standard Service (PSS) udgiver jævnligt artikler indenfor GPC/SEC Tips & Tricks sammen med LC/GC Europe i bladet LCGC Europe`s The Column.
Sidste udgivelse er “Product Registration and REACH”
GPC/SEC Tips & Tricks 04/2017
Protein Analysis with Size Exclusion Chromatography
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the standard method to separate samples by molecular size. In protein analysis, size-exclusion chromatography is either applied to detect and quantify aggregation, or to measure the complete molar mass distribution. However, method development is not trivial and the choice of suitable detection options is crucial.
Tips & Tricks – 04.2017 – D. Held and T. Hofe
Sensory – Applications corner
Alpha MOS har udsendt nye applikationer indenfor sensor testing udstyr:
10050-E-tongue evaluation of medicinal plants
Sensory – Applications corner
Alpha MOS har udsendt nye applikationer indenfor sensor testing udstyr:
10051-E-tongue-Differentiation-Chinese-Rice-Wine-based-on-age