RNA Extraction kits – 250 pcs. – COVID-19

Varenummer: VR6568-02

Beskrivelse:

Viral RNA Extraction from Respiratory Specimens – COVID-19

The EZgeneTM Viral DNA/RNA mini kit provides an easy and reliable method for isolating total viral RNA from plasma, serum, nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. This procedure has been tested for isolating nucleic acids from COVID-19, Hepatitis A, Hepatitis C and HIV. The isolated RNA can be used for PCR, qRT-PCR and other downstream applications.

Acceptable Specimens

Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable Serum, plasma, or other body fluid.

Kit Contents
Catalog# VR6568-02
Preps 250
Buffer LYE 90 mL
Proteinase K (4mg/mL)
RNA Wash Buffer * 50 mL
Buffer RB* 90 mL
L Solution 550 µL
DEPC-Treated ddH2O 15mL
Mini Column with collection tubes 250

Bruger manual VR6568

Produkterne:

V/N: VR6568-01 – 50 stk.
V/N: VR6568-02 – 250 stk.

Log ind for at se prisen

Specifikationer

LEVERANDØR

LEVERINGSTID

Antal

250

CE certifikat

Ja

Applikationstype

RNA – COVID-19

FDA certifikat

FDA registration

ISO9001 certifikat

Produkt navn

Viral RNA Extraction

Information:

Viral RNA Isolation Protocol

 

The protocol is developed for processing 50-300 μL samples.

 

  1. Pipet 20 µL Proteinase K, 2 µL L Solutionand 300 µL Buffer LYE to a 1.5 mL tube. Calculate the number of samples to be processed and make master mix of proteinase K,   L Solution and Buffer LYE.

 

  1. Pipet 300 µL nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, or plasma, serum, into the 1.5 mL tube from Step 1. Mix well by pulse-vortexting for 10 seconds. Incubate at room temperature for 10 min to lyse the cells and virus.

 

  1. Add 600 µL isopropanol and mix well by pulse-vortexing for 5 seconds. Spin briefly to collect the drop from the lid.

Note: Maintain the ratio of (Sample+ Buffer LYE): Isopropanol= 1:1

 

  1. Transfer 600 µL of the sample from step 3 into a RNA column and centrifuge at 10,000 rpm for 30 seconds. Discard the flow-through carefully to a waste container by pipetting and put the column back to the collection Transfer the remaining sample to the column and centrifuge at 10,000 rpm for 30 seconds. Discard the flow through and put the column back to the collection tube. Note: When transferring the column/collection tubes, always hold the upper rim of the collection tube, not the column, to avoid possible drop off of the collection tube.

 

  1. Add 500 µL Buffer RB to the column and centrifuge at 10,000 rm for 30 seconds. Discard the flow-through. Put the column back to the collection tube.

 

  1. Add 500 µL RNA Wash Buffer to the column and centrifuge at 10,000 rpm for 30 seconds. Discard the flow-through. Put the column back to the collection tube.

 

 

  1. Centrifuge the empty column at 12,000 rpm for 2 min. It is critical to remove residual ethanol for optimal elution.

 

  1. Transfer the RNA column to a RNase-free 5 mL tube, add 35-50 µL DEPC-treated water to the column and centrifuge at 10,000 rpm for 30 seconds. The viral RNA is in the flow-through liquid.

 

  1. Optional: Add the eluent back to the column for a second elution.

Note: The first elution normally yields 70% of the RNA while the second elution yields another 20-30% of the RNA bound to the column.

Note: The purified RNA should be put on ice for downstream application or store at -20°C.